terminal transferase kit (New England Biolabs)
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New England Biolabs
terminal transferase kit
Terminal Transferase Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/terminal+transferase/Terminal+Transferase/pm42092133-731-16-19
Average 98 stars, based on 1301 article reviews
Terminal Transferase Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/terminal+transferase/Terminal+Transferase/pm42092133-731-16-19
Average 98 stars, based on 1301 article reviews
terminal transferase kit - by Bioz Stars,
2026-10
98/100 stars
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Plasmid Preparation:Article Title: Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters Article Snippet: .. The targeting vector was linearized using restriction enzymes, and then the 3′ end of the targeting vector was biotinylated with biotin-16-ddUTP (Roche, Basel, Switzerland) using Article Title: Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters. Article Snippet: Both crRNA and tracrRNA were purchased from FASMAC, Atsugi, Japan and their sequences of the crRNAs were as follows: R26-T2-crRNA (5′-CGC CCA UCU UCU AGA AAG ACG UUU UAG AGC UAU GCU GUU UUG-3′) and PITCh-gRNA3-crRNA (5′-GCA UCG UAC GCG UAC GUG UUG UUU UAG AGC UAU GCU GUU UUG-3′). .. For the DAT/iCre knockin mice, Cas9 mRNA and single guide RNA (sgRNA) were injected together with the targeting vector into the cytoplasm of two-cell stage embryos of C57BL/6NJcl mice using a modified 2C-HR-CRISPR method.57 The targeting vector was linearized using restriction enzymes, and then the 3′ end of the targeting vector was biotinylated with biotin-16-ddUTP (Roche, Basel, Switzerland) using Incubation:Article Title: Direct-to-library methods, systems, and compositions Article Snippet: The processing without the beads (DTL w/o beads) was performed as follows: Plasma was incubated with 1.6 units of Proteinase K (Sigma-Aldrich, St. Louis, MO) in 1× Terminal Transferase Reaction buffer (NEB, Ipswich, MA) at 60° C. for 20 minutes and 95° C. for 10 minutes and placed on ice until cool. .. One third of the solution was added to the A tailing reaction containing 0.16 mM dATP (NEB, Ipswich, MA) and 20 units Article Title: Direct-to-library methods, systems, and compositions Article Snippet: .. 40.0 μL of 10× Article Title: Increased Availability of Selective Trace Elements Enhanced Anaerobic Benzoate Oxidation in Geotalea daltonii Article Snippet: RNA was extracted as previously described, treated with 0.1 M DTT, and incubated at 42 °C to eliminate secondary structure. cDNA was purified by incubation with 1 M NaOH at 65 °C for 20 min and GeneJet PCR Purification kit (Thermo Scientific, Pittsburgh, PA, USA,). .. DNA was tailed by incubation with Article Title: Direct-to-library methods, systems, and compositions Article Snippet: .. 2.0 μL of 10 mM dATP, 2.0 μL Article Title: Direct-to-library methods, systems, and compositions Article Snippet: .. The reaction conditions used were as follows: (i) RNA A-tailing reaction: 50.0 μL of each spiked sample was mixed with a mastermix comprising of 20.0 μL of 10× E. coli Poly(A) polymerase Reaction Buffer (NEB, Ipswich, MA), 6.0 μL of 10 mM ATP and 3.0 μL Poly(A) polymerase (5000 u/μL, NEB, Ipswich, MA) and 121.0 μL Nuclease-free water to prepare the A-tailing reaction which was incubated at 37° C. for 40 min. (ii) DNA A-tailing reaction: 50.0 μL of each spiked sample was mixed with a master mix comprising of 40.0 μL of 10× Knock-In:Article Title: Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters. Article Snippet: Both crRNA and tracrRNA were purchased from FASMAC, Atsugi, Japan and their sequences of the crRNAs were as follows: R26-T2-crRNA (5′-CGC CCA UCU UCU AGA AAG ACG UUU UAG AGC UAU GCU GUU UUG-3′) and PITCh-gRNA3-crRNA (5′-GCA UCG UAC GCG UAC GUG UUG UUU UAG AGC UAU GCU GUU UUG-3′). .. For the DAT/iCre knockin mice, Cas9 mRNA and single guide RNA (sgRNA) were injected together with the targeting vector into the cytoplasm of two-cell stage embryos of C57BL/6NJcl mice using a modified 2C-HR-CRISPR method.57 The targeting vector was linearized using restriction enzymes, and then the 3′ end of the targeting vector was biotinylated with biotin-16-ddUTP (Roche, Basel, Switzerland) using Injection:Article Title: Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters. Article Snippet: Both crRNA and tracrRNA were purchased from FASMAC, Atsugi, Japan and their sequences of the crRNAs were as follows: R26-T2-crRNA (5′-CGC CCA UCU UCU AGA AAG ACG UUU UAG AGC UAU GCU GUU UUG-3′) and PITCh-gRNA3-crRNA (5′-GCA UCG UAC GCG UAC GUG UUG UUU UAG AGC UAU GCU GUU UUG-3′). .. For the DAT/iCre knockin mice, Cas9 mRNA and single guide RNA (sgRNA) were injected together with the targeting vector into the cytoplasm of two-cell stage embryos of C57BL/6NJcl mice using a modified 2C-HR-CRISPR method.57 The targeting vector was linearized using restriction enzymes, and then the 3′ end of the targeting vector was biotinylated with biotin-16-ddUTP (Roche, Basel, Switzerland) using Modification:Article Title: Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters. Article Snippet: Both crRNA and tracrRNA were purchased from FASMAC, Atsugi, Japan and their sequences of the crRNAs were as follows: R26-T2-crRNA (5′-CGC CCA UCU UCU AGA AAG ACG UUU UAG AGC UAU GCU GUU UUG-3′) and PITCh-gRNA3-crRNA (5′-GCA UCG UAC GCG UAC GUG UUG UUU UAG AGC UAU GCU GUU UUG-3′). .. For the DAT/iCre knockin mice, Cas9 mRNA and single guide RNA (sgRNA) were injected together with the targeting vector into the cytoplasm of two-cell stage embryos of C57BL/6NJcl mice using a modified 2C-HR-CRISPR method.57 The targeting vector was linearized using restriction enzymes, and then the 3′ end of the targeting vector was biotinylated with biotin-16-ddUTP (Roche, Basel, Switzerland) using Lysis:Article Title: Direct-to-library methods, systems, and compositions Article Snippet: .. 2.0 μL of 10 mM dATP, 2.0 μL |
